Cleavage of Epstein-Barr virus DNA by restriction endonucleases EcoRI, HindIII and BamI
نویسندگان
چکیده
منابع مشابه
Cleavage maps for human cytomegalovirus DNA strain AD169 for restriction endonucleases EcoRI, BglII, and HindIII.
We have used cloned EcoRI fragments of the human CMV (HCMV) genome, strain AD169, to prepare restriction endonuclease maps of the DNA. Individual 32P-labeled cloned fragments were hybridized to Southern blots of HCMV DNA cleaved to completion with the restriction endonucleases BglII and HindIII and cleaved partially with EcoRI. By determining which EcoRI fragments hybridized to the same band on...
متن کاملHeterogeneity in sensitivity to cleavage by the restriction endonucleases ECORI and HindIII of circular kinetoplast DNA molecules of Crithidia acanthocephali
Kinetoplast DNA (kDNA) of the protozoan Crithidia acanthocephali consists mainly of an association of approximately 27,000 covalently closed, 0.8-micron (1.58 X 10(6) daltons) circular molecules apparently held together in a particular structural configuration by topological interlocking. The sensitivities of circular kDNA molecules to the restriction endonucleases EcoRI and HindIII have been s...
متن کاملMolecular cloning of DNA fragments produced by restriction endonucleases Sa1I and BamI.
The highly specific restriction endonucleases Sa1I and BamI produce DNA fragments with complementary, cohesive termini that can be covalently joined by DNA ligase. The Escherichia coli kanamycin resistance factor pML21 has one SalI site, at which DNA can be inserted without interfering with the expression of drug resistance or replication of the plasmid. A more convenient cloning vehicle can be...
متن کاملRole of thymidine residues in DNA recognition by the EcoRI and EcoRV restriction endonucleases.
We have synthesized a series of oligonucleotides containing the EcoRI (GAATTC) or EcoRV (GATATC) recognition site within which or adjacent to which thymidine was substituted by uridine or derivatives of uridine. The effects of these substitutions on the rate of the EcoRI and EcoRV catalyzed cleavage reaction were investigated. Our results show that most of the substitutions within the site are ...
متن کاملLymphoma infectious mononucleosis by Epstein-Barr virus: A case report
Background: Epstein-Bar virus (EBV) causes infectious mononucleosis in more than 90% of children. Rarely, mononucleosis may lead to malignancy. The aim the current study was to introduce a child with mononucleosis and persistent adenopathy. Case report: A 4-year-old male patient presented with complaints of fever, shortness of breath and sore throat. He was treated with antibiotics and prednis...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1978
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/5.4.1387